In a 5-4 decision late Friday, the high court denied an application for a partial stay in order to allow the Education Department rules to take effect, upholding multiple lower court decisions blocking enforcement of the new rule.

Earlier this year, the Department of Education under President Joe Biden announced new Title IX regulations, which were scheduled to go into effect this month. The new regulations defined sex discrimination as including discrimination on the basis of sexual orientation and gender identity.

The expansion of the definition of “sex” prompted many states to file lawsuits against the Biden administration, with concerns including the imposing trans ideology on public schools.

Last month, U.S. District Judge John Broomes, a Trump appointee, issued a ruling in which he temporarily blocked the administration from enforcing the new regulations.

He wrote, “The Final Rule would, among other things, require schools to subordinate the fears, concerns, and privacy interests of biological women to the desires of transgender biological men to shower, dress, and share restroom facilities with their female peers.” 

“Moreover, to expand sex discrimination to encompass ‘self-professed and potentially ever-changing gender identity is inconsistent with Title IX’s sex-separation dictates.'”